Defender Chaluba, 22, shook goalkeeper Wojciech Szczecin with a huge shot from close range, after a touch from Antonio Rudiger and after a corner kick by Hakim Ziyech.
The VAR dismissed allegations of handball against Rudiger.
James, 21, showed a high skill in shooting a powerful ball from a narrow angle to add the second goal in the 55th minute.
Less than three minutes later, Hudson-Odoi, 21, scored from close range, after a pass from another player from the club’s academy, Ruben Loftus-Cheek.
Substitute Werner managed to seal the goals before the final whistle after another cross pass from Ziyech.
European champions Chelsea, who lost 1-0 in Turin in September, forced their Italian opponent to appear modest, and the English team shot 20 balls compared to only four balls for its opponent, and took 12 corner kicks for three corner kicks for the visiting team.
Juventus had previously qualified for the playoffs, but the loss made them fall to second place with 12 points, the same as Chelsea’s, one round before the end of the group stage.